• HIC Analysis of Different Monoclonal Antibodies using Isopropanol as Modifier

HIC Analysis of Different Monoclonal Antibodies using Isopropanol as Modifier

Jul 24 2019 Read 839 Times

Antibodies are often analysed in HIC mode because of the non-denaturating conditions used. Organic solvents in the mobile phase can cause the denaturation of antibodies. By adding of 15 % isopropanol the denaturation can be reduced and higher recoveries are achieved. The analysis of different MAbs using YMC’s BioPro HIC BF achieved high resolution with narrow peak widths.

This application note developed by the University of Geneva (Department of Analytical Pharmaceutical Chemistry) shows how to realise a robust chromatographic method using four commercially available MAbs: Adalimumab (Humira®), Brentuximab (commercially available as antibody drug conjugate “Brentuximab-vedotin” / Adcetris®), Pembrolizumab (Keytruda®) and Reslizumab (Cinqaero®) with 15 % isopropanol as modifier can be achieved with higher recoveries.

Reader comments

Do you like or dislike what you have read? Why not post a comment to tell others / the manufacturer and our Editor what you think. To leave comments please complete the form below. Providing the content is approved, your comment will be on screen in less than 24 hours. Leaving comments on product information and articles can assist with future editorial and article content. Post questions, thoughts or simply whether you like the content.

Post a Comment

Digital Edition

Chromatography Today - Buyers' Guide 2020

December 2019

In This Edition Articles - On-column digestion of mAbs for automated middle-level analysis by LC-MS - Increasing Peak Capacity for the Gradient Analysis of Protein Digests and other Complex...

View all digital editions



Feb 03 2020 Dubai, UAE

China Lab 2020

Mar 11 2020 Guangzhou, China


Mar 16 2020 Dubai, UAE


Mar 16 2020 Dubai, UAE

ACS National Meeting & Expo, Spring 2020

Mar 22 2020 Philadelphia, PA, USA

View all events