Bioanalytical

  • HIC Analysis of Different Monoclonal Antibodies using Isopropanol as Modifier

HIC Analysis of Different Monoclonal Antibodies using Isopropanol as Modifier

Jul 24 2019 Read 631 Times

Antibodies are often analysed in HIC mode because of the non-denaturating conditions used. Organic solvents in the mobile phase can cause the denaturation of antibodies. By adding of 15 % isopropanol the denaturation can be reduced and higher recoveries are achieved. The analysis of different MAbs using YMC’s BioPro HIC BF achieved high resolution with narrow peak widths.

This application note developed by the University of Geneva (Department of Analytical Pharmaceutical Chemistry) shows how to realise a robust chromatographic method using four commercially available MAbs: Adalimumab (Humira®), Brentuximab (commercially available as antibody drug conjugate “Brentuximab-vedotin” / Adcetris®), Pembrolizumab (Keytruda®) and Reslizumab (Cinqaero®) with 15 % isopropanol as modifier can be achieved with higher recoveries.
 

Reader comments

Do you like or dislike what you have read? Why not post a comment to tell others / the manufacturer and our Editor what you think. To leave comments please complete the form below. Providing the content is approved, your comment will be on screen in less than 24 hours. Leaving comments on product information and articles can assist with future editorial and article content. Post questions, thoughts or simply whether you like the content.

Post a Comment




Digital Edition

Chromatography Today - September 2019

September 2019

In This Edition Fundamental ASpects The Quest for True Ultra High Performance Supercritical Fluid Chromatography Walk-up SFC-MS for Fast Purification of reaction Mixtures within Discover...

View all digital editions

Events

MicroTAS 2019

Oct 27 2019 Basel, Switzerland

Eastern Analytical Symposium

Nov 18 2019 Princeton, NJ, USA

HTC-16

Jan 29 2020 Ghent, Belgium

MEDLAB MIDDLE EAST

Feb 03 2020 Dubai, UAE

China Lab 2020

Mar 11 2020 Guangzhou, China

View all events