LC-MS

  • Analysis of commercial monoclonal antibodies by microLC-MS/MS

Analysis of commercial monoclonal antibodies by microLC-MS/MS

Apr 22 2020 Read 659 Times

Monoclonal antibodies (MAbs) are high-potency agents mainly used in cancer therapy or to combat auto-immune diseases. Even small amounts of these substances can have severe effects even when taken accidently.

Therefore, it is of interest to analyse different kinds of samples which could contain these antibodies, e.g. solutions prepared for patient application (usually a dilution of the dosage form). By use of microLC-MS/MS, only a very small amount of this valuable solution is required to identify and quantify the monoclonal antibody in very short run times.

In addition, microLC-MS/MS can be used to monitor residues on surfaces or materials in hospitals, pharmacies or laboratories that prepare solutions for patient application. An easy way to monitor residues is by wipe sampling followed by microLC-MS/MS. This workflow could be applied to MAbs as well.

The ideal selectivity of YMC-Triart Bio C4 for intact monoclonal antibodies has been presented already in several applications. In this application note, YMC-Triart Bio C4 was used in YMC capillary column format to analyse and identify intact commercial MAbs, such as rituximab or bevacizumab, by microLC-MS/MS. An elevated temperature of 75°C and formic acid as additive was applied.

Reader comments

Do you like or dislike what you have read? Why not post a comment to tell others / the manufacturer and our Editor what you think. To leave comments please complete the form below. Providing the content is approved, your comment will be on screen in less than 24 hours. Leaving comments on product information and articles can assist with future editorial and article content. Post questions, thoughts or simply whether you like the content.

Post a Comment





Digital Edition

Chromatography Today - June 2020

June 2020

In This Edition Articles Fundamental Aspects - Separation of the 4 Enantiomers of the Fungicide Spiroxamine by LC-MS/MS - Optimising the Chiral Separation of the Pesticide Diniconazole...

View all digital editions

Events

Mass Spec 2020

Sep 14 2020 Virtual Symposium

ISC 2020

Sep 21 2020 Budapest, Hungary

MSB 2020 - NEW DATES

Sep 27 2020 Saint-Malo, France

CE Pharm 2020 - ONLINE EVENT

Sep 28 2020 Bethesda, MD, USA

MicroTAS 2020

Oct 04 2020 ONLINE EVENT

View all events