• Researchers attempt to find candidate protein markers for tumor sensitivity to L-OHP

Bioanalytical

Researchers attempt to find candidate protein markers for tumor sensitivity to L-OHP

Dec 30 2011

Intracellular S100A10 protein expression levels in drug-untreated CRC cells differ according to cell lines and are significantly correlated with sensitivity of CRC cells L-OHP exposure, it has been found by researchers.

In an attempt to find candidate protein markers for tumor sensitivity to L-OHP from intracellular proteins of human colorectal cancer (CRC) cell lines, the researchers performed expression difference mapping (EDM) analysis of whole cell lysates from 11 human CRC cell lines with different sensitivities to L-OHP by using surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF MS), and identified a candidate protein by liquid chromatography/mass spectrometry ion trap time-of-flight (LCMS-IT-TOF).

The results showed that of the qualified mass peaks obtained by EDM analysis, 41 proteins were differentially expressed in 11 human colorectal cancer cell lines. Among these proteins, the peak intensity of 11.1 kDa protein was strongly correlated with the L-OHP sensitivity (50% inhibitory concentrations) (P <0.001, R2 = 0.80).

Protein S100-A10 (S100A10) was identified by MS/MS ion search using LCMS-IT-TOF. The differential expression was verified, and the correlation between S100A10 protein expression levels in drug-untreated CRC cells and their L-OHP sensitivities by Western blot analyses. In addition, S100A10 protein expression levels were not correlated with sensitivity to 5-fluorouracil, suggesting that S100A10 is more specific to L-OHP than to 5-fluorouracil in CRC cells. S100A10 was detected in cell culture supernatant, suggesting secretion out of cells.


Digital Edition

Chromatography Today - Buyers' Guide 2022

October 2023

In This Edition Modern & Practical Applications - Accelerating ADC Development with Mass Spectrometry - Implementing High-Resolution Ion Mobility into Peptide Mapping Workflows Chromatogr...

View all digital editions

Events

NGVS 2024

May 23 2024 Beijing, China

Analiza

May 28 2024 Tel Aviv, Israel

HTC-18

May 28 2024 Leuven, Belgium

PREP 2024

May 28 2024 Philadelphia, PA, USA

CISILE 2024

May 29 2024 Beijing, China

View all events