In this contribution we provide insight into the functional principles of flow field flow fractionation (F4) which enables the user to separate macromolecules and particles with high efficiency. The latest development in this field, the hollow fibre technology (HF5), will be introduced. Based on several exemplary measurements we elucidate the advantages of HF5: it provides higher sensitivity compared to SEC and AF4, better separation performance for certain delicate samples (e.g.: antibodies) and increased versatility through the use of a disposable HF5 cartridge, which can be changed within seconds, a new feature that helps to avoid cross contamination and sterility issues.
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