Electrophoretic Separations

Hollow Fibre Flow Field Flow Fractionation (HF5) Increases Sensitivity and Efficiency in the Separation of Proteins and Complex Protein Mixtures

May 30 2012

Author: by Stephan Elsenberg, Thomas Jocks, Christoph Johann on behalf of Wyatt Technology Corporation

Free to read

This article has been unlocked and is ready to read.


In this contribution we provide insight into the functional principles of flow field flow fractionation (F4) which enables the user to separate macromolecules and particles with high efficiency. The latest development in this field, the hollow fibre technology (HF5), will be introduced. Based on several exemplary measurements we elucidate the advantages of HF5: it provides higher sensitivity compared to SEC and AF4, better separation performance for certain delicate samples (e.g.: antibodies) and increased versatility through the use of a disposable HF5 cartridge, which can be changed within seconds, a new feature that helps to avoid cross contamination and sterility issues.

Free to read

This article has been unlocked and is ready to read.


Digital Edition

Chromatography Today - Buyers' Guide 2022

October 2023

In This Edition Modern & Practical Applications - Accelerating ADC Development with Mass Spectrometry - Implementing High-Resolution Ion Mobility into Peptide Mapping Workflows Chromatogr...

View all digital editions


MSB 2024

May 19 2024 Brno, Czech Republic

Water Expo Nigeria 2024

May 21 2024 Lagos, Nigeria

NGVS 2024

May 23 2024 Beijing, China


May 28 2024 Tel Aviv, Israel


May 28 2024 Leuven, Belgium

View all events