LC-MS

  • UHPLC Analysis of benzodiazepines and their metabolites by LC-MS/MS

UHPLC Analysis of benzodiazepines and their metabolites by LC-MS/MS

Sep 24 2019

The analysis of benzodiazepines in biological fluids is important to clinicians and forensic toxicologists because of their sedative, hypnotic, antianxiety, antiepileptic and muscle relaxant properties. However, it is challenging to analyse these compounds because their chemical structures, molecular weights and fragmentation patterns during mass spectrometry are very similar.

This application note shows a high-resolution UHPLC separation using a robust YMC-Triart C18 column including the previous sample preparation. The simultaneous analysis of etizolam, triazolam, and their metabolites 8-ethylhydroxyetizolam, alpha-hydroxyetizolam, 4-hydroxytriazolam and alpha-hydroxytriazolam in biological samples is performed by MS/MS detection.


Digital Edition

Chromatography Today - March 2021

March 2021

In This Edition Modern & Practical Applications - Advancing Effective Glycan Analysis - Delivering the Power of Ion Mobility Spectrometry - Mass Spectrometry to the Point of Analysis - The...

View all digital editions

Events

EuroAnalysis 2021 - cancelled

Aug 22 2021 Nijmegen, Netherlands

ACS National Meeting & Expo, Fall 2021

Aug 22 2021 Atlanta, GA, USA & Virtual

Microscopy Conference 2021

Aug 22 2021 Digital event

Labvolution 2021 - NEW DATES

Sep 07 2021 Digital event

BMSS Annual Meeting

Sep 08 2021 Sheffield, UK & Online

View all events