• UHPLC Analysis of benzodiazepines and their metabolites by LC-MS/MS

LC-MS

UHPLC Analysis of benzodiazepines and their metabolites by LC-MS/MS

Sep 24 2019

The analysis of benzodiazepines in biological fluids is important to clinicians and forensic toxicologists because of their sedative, hypnotic, antianxiety, antiepileptic and muscle relaxant properties. However, it is challenging to analyse these compounds because their chemical structures, molecular weights and fragmentation patterns during mass spectrometry are very similar.

This application note shows a high-resolution UHPLC separation using a robust YMC-Triart C18 column including the previous sample preparation. The simultaneous analysis of etizolam, triazolam, and their metabolites 8-ethylhydroxyetizolam, alpha-hydroxyetizolam, 4-hydroxytriazolam and alpha-hydroxytriazolam in biological samples is performed by MS/MS detection.


Digital Edition

Chromatography Today - Buyers' Guide 2022

October 2023

In This Edition Modern & Practical Applications - Accelerating ADC Development with Mass Spectrometry - Implementing High-Resolution Ion Mobility into Peptide Mapping Workflows Chromatogr...

View all digital editions

Events

Korea Lab 2024

Apr 23 2024 Kintex, South Korea

Korea Chem 2024

Apr 23 2024 Seoul, South Korea

AOCS Annual Meeting & Expo

Apr 28 2024 Montreal, Quebec, Canada

SETAC Europe

May 05 2024 Seville, Spain

ChemUK 2024

May 15 2024 Birmingham, UK

View all events