New Size Exclusion Columns for UHPLC
May 09 2016
TSKgel UP-SW3000, a new two micron stationary phase, is the latest addition to the renowned TSKgel SW series of silica based gel filtration columns from Tosoh Bioscience. Packed into UHPLC column hardware it supports a smooth transfer of existing HPLC methods to UHPLC technology. TSKgel UP-SW3000 features the same surface modification and pore size as the well-established TSKgel G3000SWXL being the gold standard for QC of therapeutic antibodies in Biopharma for decades. Over 40 years of experience in developing and producing SEC stationary phases are incorporated into the new series of UHPLC columns for the analysis of proteins in the range of 10 to 500 kDa.
Methods developed on conventional gel filtration columns can easily be transferred to UHPLC technology when using TSKgel UP-SW3000. The 30cm column delivers increased resolution between mAb fragments, monomers, and aggregates. A 15cm column is available to shorten analysis time while maintaining resolution. To achieve maximum resolution and fully exploit the potential of the columns the use of a UHPLC system is highly recommended. A so-called DC (direct connect) guard column can be attached directly to the analytical column to minimise extra column band broadening.
TSKgel SW columns stand out from other silica-based high performance size exclusion columns by virtue of their large pore volumes. They are based on highly porous, ultra-pure silica particles, the surface of which has been shielded from interacting with proteins by applying a proprietary surface chemistry. This ensures highest recoveries and accurate quantitation. The validated manufacturing and packing process delivers a reliable batch-to-batch reproducibility.
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