• Quick Evaluation of mAb Glycoforms and ADCC Activity


Quick Evaluation of mAb Glycoforms and ADCC Activity

Nov 05 2020

TSKgel FcR-IIIA-NPR, a new HPLC column developed by Tosoh Bioscience, can provide valuable information on antibody biologic activity and glycoforms in many phases of development and production. This new affinity column is based on a recombinant Fc gamma IIIa receptor ligand immobilised on a non-porous polymer particle. 

Fc gamma IIIa receptor plays a key role in antibody-dependent cell mediated cytotoxicity (ADCC) which is a crucial mechanism of action of anti-tumour mAbs. These Fc-mediated effector functions are known to be related to Fc-glycans of antibodies. Hence, separation patterns of therapeutic antibodies on TSKgel FcR-IIIA-NPR can be correlated to both glycan pattern and the ADCC activity of immunoglobulins. Early eluting peaks of TSKgel FcR-IIIA-NPR represent glycoforms with low ADCC activity while late eluting peaks represent glycoforms with high ADCC activity. 

The rapid thirty-minute separation allows the analysis of large numbers of mAb samples to gain valuable first information on the distribution of glycoforms and expected ADCC activity. This fast and efficient method can be applied to purified samples and supernatant alike and can therefore be used in many phases of development and production such as cell line screening in early R&D, biosimilar/originator comparison, upstream development and optimisation, monitoring of glycoengineering, or lot-to-lot comparison in QC.

More information online

Digital Edition

Chromatography Today - Buyers' Guide 2022

October 2023

In This Edition Modern & Practical Applications - Accelerating ADC Development with Mass Spectrometry - Implementing High-Resolution Ion Mobility into Peptide Mapping Workflows Chromatogr...

View all digital editions


Medlab Middle East

Feb 05 2024 Dubai, UAE

H2 Forum

Feb 19 2024 Berlin, Germany


Feb 24 2024 San Diego, CA, USA

China Lab 2024

Mar 05 2024 Guangzhou, China

View all events