Speed up your oligonucleotide analysis – in under 2 Minutes!

Precise and rapid analysis of oligonucleotides is essential for modern production workflows. Reliable and efficient quality control helps maintain consistent product quality while keeping development and manufacturing processes on schedule. This Application Note demonstrates how a six-oligonucleotide mixture (12–33mer) can be fully resolved using a 30 mm YMC Accura BioPro IEX QF column at 70 °C, delivering high-resolution chromatographic results in under two minutes.

Despite its very short column length, the method provides excellent separation performance. The compact format supports ultra-fast analysis without compromising resolution, making it ideal for high-throughput laboratories. The bioinert Accura hardware, combined with the robust BioPro IEX QF resin, ensures reliable peak shapes and minimizes unwanted interactions that can occur when analysing sensitive oligonucleotides. This inert design also reduces carry-over, contributing to consistent and reproducible workflows.

Operating the column at 70 °C offers a significant advantage: elevated temperatures reduce the formation of secondary structures within the oligonucleotides. As a result, peak shapes are sharper, efficiency increases, and overall separation performance improves. This makes the method particularly useful for laboratories working with complex or structure-prone oligonucleotides, where peak broadening and poor resolution are common challenges.

In summary, the method offers a combination of speed, stability, and precision that is highly beneficial for modern oligonucleotide analytics. Key advantages include:

• Analysis times below two minutes, ideal for high sample throughput
• Accurate and reliable separation of six oligonucleotides, even with a 30 mm column
• Enhanced peak sharpness due to elevated temperature operation
• Bioinert hardware, minimizing adsorption and ensuring long-term reproducibility
• Robust method transferability, as high-temperature operation reduces variability across instruments and laboratories

The YMC Accura BioPro IEX QF column is therefore a powerful solution for routine oligonucleotide analysis, supporting efficient workflows in research, development, and large-scale production. Its combination of thermal stability, bioinertness, and superior separation efficiency ensures high-quality results—even under the tight time constraints of fast analytical cycles.