BioPro IEX SmartSep + YMC glass columns = perfect match

In oligonucleotide purification, success is not defined by a single component, but by the integrated system of resin and hardware. By combining BioPro IEX SmartSep with YMC glass columns, you obtain a setup that is scientifically robust and process-ready: maximum resolution, temperature-stable method performance, and reliable scalability from early development through pilot scale.

BioPro IEX SmartSep – AEX for efficient oligo purification

BioPro IEX SmartSep is a strong anion exchange resin with small particle sizes, designed to achieve high resolution even when shortmers (N–1) and longmers (N+1) needs to be separated. Across DNA, RNA and 2'-OMe-RNA, the resin consistently provides stable retention, symmetric peak shapes and reproducible separation of backbone types such as phosphorothioate (PS) and phosphodiester (PO).

Temperature is often an important tool for enhancing resolution. With true thermal stability up to 60 °C, BioPro IEX SmartSep allows temperature to be used as an additional separation parameter without peak drift or loss of binding capacity. This enables the fine selectivity and robustness required for modern AEX-based oligo purification.

YMC glass columns – reliable hardware for oligo AEX

The performance of an AEX resin is closely linked to the column hardware. Metal-free YMC glass columns enable precise, reproducible packing that preserves resin efficiency and delivers sharp separations – essential for fractionating closely related oligo species.

Depending on your scale and process goals, YMC offers the ideal glass column solution. For applications using particles between 10 and 30 µm, the ECO^PLUS column line delivers high efficiency and is perfectly suited for screening and method development. If higher temperatures are required for your purification, the YMC HT column line with reliable stability up to 70 °C provides the ideal solution. And once you move into the pilot scale, the YMC Pilot^PLUS columns offer a seamless transition toward production - featuring scalable, GMP-compliant hardware that ensures reproducible packing performance.

High-Resolution Oligo Purification by Design

The combination of BioPro IEX SmartSep and YMC glass columns addresses the demands of oligonucleotide purification by uniting high resolving power and thermal robustness with metal-free, chemically resistant hardware that delivers excellent packing quality. The result is efficient, reliable, and more sustainable oligo purification.

Benefit from:

• Reproducible and excellent packing quality for consistent retention and resolution over many cycles.
• High chemical and mechanical robustness of both hardware and resin, compatible with high-salt buffers and standard cleaning/sanitization media.
• Minimal risk of non-specific binding thanks to metal-free hardware and the hydrophilic base particle.
• Scalable YMC glass columns from lab to pilot scale, paired with scalable BioPro IEX for seamless method transfer across development stages.
• Temperature-robust resin and hardware to support higher resolution when needed.

No time for packing yourself?

With YMC’s packing service, you receive prepacked glass columns with reproducible quality and certified efficiency – immediately ready for oligonucleotide purification. 

For more information about YMC glass columns, please visit the website. Interested in BioPro IEX? Then take a look here.