HPLC, UHPLC
Published over 16 years ago. See the latest and most current information on HPLC, UHPLC.
The use of temperature as a tool for method development is gaining interest. In most of the reversed-phase LC methods the selectivity can be altered significantly by changing the temperature. Since temperature is an instrumental parameter it is easier to change than e. g. buffer pH and is more robust. This note demonstrates the potential of temperature variations for controlling the selectivity in LC method development.
Additionally, the features of a silica based and a polymeric stationary phase for elevated temperature LC are highlighted.
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