Drug Bioanalysis by LC-MS: some pragmatic solutions to commonly occurring problems

Oct 15 2009

Author: Gregg A Imrie, W John Lough and Terry A G Noctor on behalf of Unassigned Independent Article

Free to read

This article has been unlocked and is ready to read.


The use of atmospheric pressure ionisation (API) LC-MS/MS has been very successful in drug bioanalysis. Despite this, many challenges, such as those presented by (a) sample matrix and mobile phase effects on ionisation, and (b) the determination of endogeneous analytes, remain. An aim was therefore set to investigate these problems with a view to attempting to find simple but effective solutions to them. It was found that late-eluting interferences could be dealt with by a change of mode of ionisation, that an order of magnitude improvement in limits of detection could be had by the post-column addition of an appropriate solvent and that ion enhancement could be induced by surfactants. In the determination of endogeneous analytes it was demonstrated that the use of non-matrix calibration standards and the use of a surrogate matrix were both useful approaches.

Free to read

This article has been unlocked and is ready to read.


Digital Edition

Chromatography Today - Buyers' Guide 2022

October 2023

In This Edition Modern & Practical Applications - Accelerating ADC Development with Mass Spectrometry - Implementing High-Resolution Ion Mobility into Peptide Mapping Workflows Chromatogr...

View all digital editions


HPLC 2024

Jul 20 2024 Denver, CO, USA

ICMGP 2024

Jul 21 2024 Cape Town, South Africa

ACS National Meeting - Fall 2024

Aug 18 2024 Denver, CO, USA

JASIS 2024

Sep 04 2024 Chiba, Tokyo, Japan

BMSS-BSPR Super Meeting 2024

Sep 04 2024 University of Warwick, Coventry, UK

View all events