Kromasil HILIC HPLC Columns
Oct 25 2012
AkzoNobel introduces a HILIC phase to its Kromasil HPLC column assortment. Kromasil HILIC-D is designed for the best selectivity of polar compounds and offers a column that is 100% MS compatible. The phase is low bleed and the solvents used when running your HPLC in HILIC mode are optimal for Mass Spectrometry detection. Therefore the sensitivity can be 100 times better when using Kromasil HILIC-D over traditional RP columns.
Traditionally, polar compounds like organic acids, nucleobases, and water soluble vitamins have been a challenge to separate on a standard reversed phase column like C18. Kromasil HILIC-D offers a phase that has an alternative selectivity when compared to a C18 column, normally giving an opposite elution order. The diol derivatized Kromasil HILIC-D provides excellent reproducibility when compared to HILIC columns based on standard bare silica.
When purifying polar compounds, Kromasil HILIC-D can be scaled up, offering the same great performance as in analytical scale. Take advantage of the generous surface area which can provide high loading capacity.
Chromatographic conditions in attached figure: column size: 4.6 × 150 mm, mobile Phase: acetonitrile/water (90/10), flow rate: 1 ml/min, detection: UV @ 254 nm
Further product information can be found on the Kromasil website.
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