• Liquid chromatography 'overcomes incompatibilities' between 2-DE and proteins
    Liquid chromatography is a useful complementary technique to 2-DE, it is suggested


Liquid chromatography 'overcomes incompatibilities' between 2-DE and proteins

Nov 17 2010

The analysis of certain proteins using two-dimensional electrophoresis (2-DE) requires the complementary technique of liquid chromatography, according to researchers writing in Proteome Science.

A team from the Universite Lille Nord de France, the Universite d'Artois and the Institut de Medecine Predictive et de Recherche Therapeutique explain why 2-DE alone is not always enough to yield usable results.

"Complementary approaches are often needed to overcome incompatibilities between the 2-DE separation technique and the physical-chemical properties of certain proteins of interest," they write.

In their own research, they were looking into the role that brain capillary endothelial cells play in the blood-brain barrier, where they are the main physiological base.

They were able to elaborate a reference proteome, an advancement they say could represent a step towards full gene expression databases for capillary endothelial cells with blood-brain barrier characteristics.

Proteome Science welcomes research findings covering all issues relating to both structural and functional proteomics.

Digital Edition

Chromatography Today - Buyers' Guide 2022

October 2023

In This Edition Modern & Practical Applications - Accelerating ADC Development with Mass Spectrometry - Implementing High-Resolution Ion Mobility into Peptide Mapping Workflows Chromatogr...

View all digital editions


NGVS 2024

May 23 2024 Beijing, China


May 28 2024 Tel Aviv, Israel


May 28 2024 Leuven, Belgium

PREP 2024

May 28 2024 Philadelphia, PA, USA


May 29 2024 Beijing, China

View all events