LCMS used in lipopolysaccharide investigation

Liquid chromatography mass spectrometry (LCMS) has been used in an investigation into lipopolysaccharide-induced inflammation and additive inhibitory effect of cefmetazole.

In a study published by journal Fluids and Barriers of the CNS, scientists from the Graduate School of Medicine and Pharmaceutical Sciences in Japan sought to examine the effect of lipopolysaccharide (LPS)-induced inflammation on cerebral prostaglandin E2 (PGE2) elimination from the brain, and whether antibiotics further inhibit PGE2 elimination in LPS-treated mice.

The team noted that PGE2 is eradicated from the brain via the blood-brain barrier (BBB) in mice, although this process is inhibited by intracerebral or intravenous pre-administration of anti-inflammatory drugs and antibiotics.

They found that intracerebral or intravenous pre-administration of cefazolin had little effect on PGE2 elimination in LPS-treated mice, which indicates that peripheral administration of cefmetazole inhibits PGE2 elimination across the BBB in LPS-treated mice.

PGE2 elimination across the BBB is weakened in an LPS-induced mouse model of inflammation.