HPLC, UHPLC
Algorithm created for LC-MS datasets
Nov 16 2011
Scientists have created an algorithm to aid the analysis of multiple liquid chromatography-mass spectrometry (LC-MS) datasets.
Researchers from the Department of Electrical and Computer Engineering at the University of Texas at San Antonio, USA, suggested that identifying corresponding features, such as LC peaks registered by identical peptides, plays a crucial role in the analysis of peptides and protein mixtures so more needs to be done to enhance the efficiency of the process.
In a study published by the BMC Bioinformatics journal, the team proposed a Statistical Corresponding Feature Identification Algorithm (SCFIA) "based on both elution time shifts and peak shape correlations between corresponding features".
The scientists then tested the model, and the more convention warping function based methods, on publicly available datasets and found that both processes had an accuracy of 90 per cent.
They also found that corresponding features in multiple datasets were found in 90 per cent of peptides identified by Tandem MS.
Posted by Fiona Griffiths
Digital Edition
Chromatography Today - Buyers' Guide 2022
October 2023
In This Edition Modern & Practical Applications - Accelerating ADC Development with Mass Spectrometry - Implementing High-Resolution Ion Mobility into Peptide Mapping Workflows Chromatogr...
View all digital editions
Events
May 15 2024 Birmingham, UK
May 19 2024 Brno, Czech Republic
May 21 2024 Lagos, Nigeria
May 23 2024 Beijing, China
May 28 2024 Tel Aviv, Israel