Phosphopeptide and Non-Phosphorylated Peptide Purification and Enrichment

The extraction and enrichment of phosphopeptides from protein digests is of key importance for their successful characterisation by MALDI-MS and LC-MS analyses. The titanium dioxide (Titansphere) phase from GL Sciences has proved to be particularly effective in this role, showing increased selectivity compared with traditional IMAC methodologies.

In addition to the supply of Titansphere bulk material, designed for the enrichment of phosphopeptides from small sample volumes, GL Sciences have introduced a Phos-TiO Kit for even more effective operation.  The use of this kit enables excellent recovery rates and selectivity to be achieved. An operation manual is provided in each kit. Key benefits of Phos-TiO Kits include: high selectivity (reduces non-specific adsorption), high throughput (96-well format available), easy to operate (4 step process, 40 mins operation time) and high capacity (optimised surface activity).

MonoTips -  for the purification and enrichment of peptides and proteins

In addition to the Titansphere tips for the extraction of phosphopeptides, GL Sciences also offer a range of MonoTip pipette tips for the purification and enrichment of femtomole to micromole levels of peptides and proteins prior to MALDI-MS and LC-MS anaylysis. The monolithic silica in these tips consists of a unique double pore structure with a network of throughpores and smaller mesopores, resulting in a low pressure drop and strong analyte interaction.

Three varieties are available:

1. MonoTip C18 for desalting and enrichment of peptides and proteins
2. MonoTip Trypsin for fast trypsin digestion
3. MonoTip TiO for phosphopeptide enrichment
    

GL-Tips - for desalting and enriching peptides

For desalting and enriching peptides before introduction to the MS, GL Sciences offer GL-Tips. These are based on either SDB (styrene-divinylbenzene copolymer) or GC (graphite carbon) and retain more hydrophobic and hydrophilic peptides respectively than C18 based tips.

If you would like any further information on GL Sciences Titansphere, Phos-TiO, MonoTip or GL-Tip products please contact Hichrom on tel: 0118 930 3660 or email us.