• Save Hours of Dry-Down Time for Your Prep HPLC Fractions


Save Hours of Dry-Down Time for Your Prep HPLC Fractions

Dec 21 2009

New Anachem/Gilson Fraction Trapping FT-LC systemallows you to remove water, desalt and re-format your Prep HPLC Fractions in <15minutes.

The speed of purification and level of purity obtained with Preparative High Performance Liquid Chromatography (Prep HPLC) has increased dramatically over the last 20 years such that it is no longer a bottleneck in the pharmaceutical development cycle. However, delays have shifted downstream to the time taken to recover the purified product.

Whilst a prep HPLC run can be completed in less than 15 minutes, it can take 8-24 hours to recover the purified compound, dry it, reconstitute it in the desired solvent and format it into a compound library. This is due to the
usually high water composition of the collected fraction, the potential need to change the product structure and remove compounds such as TFA, plus re-formatting the compound into another solvent or structural form.

Now, with Anachem’s new FT-LC system this bottleneck is virtually eliminated. This system has been developed around the industry standard Gilson Prep HPLC platform, a unique specifically designed trapping column technology, FlowTrap™ from Varian Inc. and uses application specific software.

The FT-LC system can dramatically reduce the water content of fractions collected from the prep HPLC system, from 88% to less than 1% in under 15 minutes. Included in the process is a step to automatically
convert compounds from acid to base form. Recoveries have been consistent with findings in prep HPLC of > 90% for the whole process and it has already been used and verified by several major pharmaceutical companies.

Anachem is the UK & Ireland market leading specialist distributor, serving the instrumentation life science research market for over 35 years. As Gilson’s authorized dealer it offers a comprehensive range of
innovative chromatography and automated liquid handling products of the highest quality.

Contact Anachem at Anachem House, Charles Street, Luton, Bedfordshire, LU2 0EB, UK. Tel: 01582 747501, Fax: 01582 745115, Email: customersupport@anachem.co.uk, Web: www.anachem.co.uk

Digital Edition

Chromatography Today - Buyers' Guide 2022

October 2023

In This Edition Modern & Practical Applications - Accelerating ADC Development with Mass Spectrometry - Implementing High-Resolution Ion Mobility into Peptide Mapping Workflows Chromatogr...

View all digital editions


Water Expo Nigeria 2024

May 21 2024 Lagos, Nigeria

NGVS 2024

May 23 2024 Beijing, China


May 28 2024 Tel Aviv, Israel


May 28 2024 Leuven, Belgium

PREP 2024

May 28 2024 Philadelphia, PA, USA

View all events