Preparative

The Challenge of Using Small Particle Packing Materials in SEC/GPC Risks and Possibilities

Dec 08 2011

Author: T. Hofe and J. McConville on behalf of PSS Polymer Standards Service GmbH

Free to read

This article has been unlocked and is ready to read.

Download

About 5 years ago, [1] the particle size of packing media in HPLC columns was reduced to 2 µm or smaller. This new column technology named U-HPLC or UPLC offers much better resolution and column efficiency compared to standard 5 µm packing materials. For SEC/GPC, the 2 µm range can also be quite interesting but very challenging as well. In contrast to HPLC, SEC mostly deals with very large molecules. The diameter of these large molecules can easily be in the range of the interstitial volume of the 2 µm particles.

Free to read

This article has been unlocked and is ready to read.

Download


Digital Edition

Chromatography Today - Buyers' Guide 2022

October 2023

In This Edition Modern & Practical Applications - Accelerating ADC Development with Mass Spectrometry - Implementing High-Resolution Ion Mobility into Peptide Mapping Workflows Chromatogr...

View all digital editions

Events

NGVS 2024

May 23 2024 Beijing, China

Analiza

May 28 2024 Tel Aviv, Israel

HTC-18

May 28 2024 Leuven, Belgium

PREP 2024

May 28 2024 Philadelphia, PA, USA

CISILE 2024

May 29 2024 Beijing, China

View all events