The Challenge of Using Small Particle Packing Materials in SEC/GPC Risks and Possibilities
Dec 08 2011
Author: T. Hofe and J. McConville on behalf of PSS Polymer Standards Service GmbH
About 5 years ago,  the particle size of packing media in HPLC columns was reduced to 2 µm or smaller. This new column technology named U-HPLC or UPLC offers much better resolution and column efficiency compared to standard 5 µm packing materials. For SEC/GPC, the 2 µm range can also be quite interesting but very challenging as well. In contrast to HPLC, SEC mostly deals with very large molecules. The diameter of these large molecules can easily be in the range of the interstitial volume of the 2 µm particles.
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