Preparative

The Challenge of Using Small Particle Packing Materials in SEC/GPC Risks and Possibilities

Dec 08 2011

Author: T. Hofe and J. McConville on behalf of PSS Polymer Standards Service GmbH

Free to read

This article has been unlocked and is ready to read.

Download

About 5 years ago, [1] the particle size of packing media in HPLC columns was reduced to 2 µm or smaller. This new column technology named U-HPLC or UPLC offers much better resolution and column efficiency compared to standard 5 µm packing materials. For SEC/GPC, the 2 µm range can also be quite interesting but very challenging as well. In contrast to HPLC, SEC mostly deals with very large molecules. The diameter of these large molecules can easily be in the range of the interstitial volume of the 2 µm particles.

Free to read

This article has been unlocked and is ready to read.

Download


Digital Edition

Chromatography Today - Buyers' Guide 2022

December 2021

In This Edition Modern & Practocal Applications - Accelerating ADC Development with Mass Spectrometry - Implementing High-Resolution Ion Mobility into Peptide Mapping Workflows Chromatogr...

View all digital editions

Events

Lab Asia 2023

Oct 10 2023 Hybrid event

MicroTAS 2023

Oct 15 2023 Katowice, Poland

ISPPP 2023

Nov 05 2023 Vienna, Austria

SETAC North America

Nov 12 2023 Louisville, KY, USA

Eastern Analytical Symposium

Nov 13 2023 Plainsboro, NJ, USA

View all events