• Polyclonal antibody with avidity to ROS used for determination for ROS in plasma
    IgG fractions were isolated and purified through avidity chromatography on protein A column

Solid Phase Extraction (SPE)

Polyclonal antibody with avidity to ROS used for determination for ROS in plasma

Jul 05 2011

The results of a study to find the determination of ROS in plasma have been revealed.

Published in the Chemistry Central Journal 2011, the experiment used a polyclonal antibody with high avidity and to ROS.

It was then used in the development of highly sensitive ELISA for determination of ROS in plasma.

A number of eight-week old New Zealand white rabbits were immunised with the ROS-KLH conjugate and were then monitored by direct ELISA using ROS-BSA immobilised onto microwell plates as a solid phase.

Sera was collected from the rabbit with the highest antibody titer and avidity to ROS and the IgG fraction was isolated and purified through avidity chromatography on protein A column.

The conclusion revealed that the purified antibody showed high avidity to ROS; IC50= 0.4ng/ml, with the concentration of ROS in the sample justified by its capacity to stop the binding of the anti-ROS antibody to the immobilised ROS-BSA.

Rosuvastatin is often used to treat high cholesterol issues, while also plays a part in limiting cardiovascular problems. 

Digital Edition

Chromatography Today - Buyers' Guide 2022

December 2021

In This Edition Modern & Practocal Applications - Accelerating ADC Development with Mass Spectrometry - Implementing High-Resolution Ion Mobility into Peptide Mapping Workflows Chromatogr...

View all digital editions

Events

Plastics Recycling World Expo Europe

Jun 14 2023 Essen, Germany

HPLC 2023

Jun 18 2023 Dusseldorf, Germany

SinS

Jul 04 2023 Cardiff, UK

Water Expo Nigeria 2023

Jul 11 2023 Lagos, Nigeria

ACS National Meeting & Expo, Fall 2023

Aug 13 2023 San Francisco, CA, USA

View all events