The results of a study to find the determination of
ROS in plasma have been revealed.
Published in the Chemistry Central Journal 2011, the experiment used a
polyclonal antibody with high avidity and to
ROS.
It was then used in the development of highly sensitive ELISA for determination of
ROS in plasma.
A number of eight-week old New Zealand white rabbits were
immunised with the
ROS-KLH conjugate and were then monitored by direct ELISA using
ROS-BSA immobilised onto
microwell plates as a solid phase.
Sera was collected from the rabbit with the highest antibody titer and avidity to
ROS and the
IgG fraction was isolated and purified through avidity chromatography on protein A column.
The conclusion revealed that the purified antibody showed high avidity to
ROS;
IC50=
0.4ng/ml, with the concentration of
ROS in the sample justified by its capacity to stop the binding of the
anti-ROS antibody to the
immobilised ROS-BSA.
Rosuvastatin is often used to treat high cholesterol issues, while also plays a part in limiting cardiovascular problems.
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