HPLC, UHPLC
New Technical Note: High Temperature Stability of BioPro IEX SmartSep Resins
Aug 22 2023
Ion exchange chromatography (IEX) is a well-established method for the purification of biomolecules. The optimisation of the used method is very important to maximise the process productivity. BioPro IEX SmartSep resins can be used up to 60°C - offering great flexibility in method development. This is particularly advantageous for the purification of oligonucleotides.
Investigation of High Temperature Stability with Protein Samples
The examples shown in the Technical Note point demonstrate the extended temperature stability of the BioPro IEX SmartSep resins. In a first example, the BioPro IEX SmartSep S20 and Q20 resins were flushed with a buffer at 60 °C for 20 hours. The column performance test showed no retention time shift for the proteins investigated – even after repeating the procedure for 5 times! Furthermore, no change in the separation or peak distortion could be observed for BioPro IEX SmartSep Q and S resins even after 100 hours of exposure to a 60 °C solution.
More Practical Example for the Purification of Oligonucleotides
Even under more practical conditions for the purification of oligonucleotides the BioPro IEX SmartSep Q resin maintained its performance. The elution profile of an antisense oligonucleotide remained constant even after eluting the column at 60°C for 130 runs. There is no retention time shift, and the column efficiency remains the same.
Conclusion
These experiments demonstrate the excellent temperature stability of the BioPro IEX SmartSep resins. The resins maintain their performance when used with higher eluent temperatures. This can be useful for many applications such as oligonucleotide purifications and offer a greater flexibility in method development.
Find all these useful information about high temperature stability of BioPro IEX SmartSep resin in this Technical Note.
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