• New Technical Note: High Temperature Stability of BioPro IEX SmartSep Resins
    Figure 1: Result of the column performance test for BioPro IEX SmartSep S20. The column was flushed with 20 mM NaH2PO4-Na2HPO4 (pH 6.8) at 60 °C for 20 h. Then a column performance test at 25 °C was performed. This was repeated for five times.
  • Figure 2: Result of the column performance test for BioPro IEX SmartSep Q20. The column was flushed with 20 mM Tris-HCl (pH 8.1) at 60 °C for 20 h. Then a column performance test at 25 °C was performed. This was repeated for five times.
  • Figure 3: More practical example of the purification of antisense oligonucleotide using BioPro IEX SmartSep Q resin at 60°C.

HPLC, UHPLC

New Technical Note: High Temperature Stability of BioPro IEX SmartSep Resins

Aug 22 2023

Ion exchange chromatography (IEX) is a well-established method for the purification of biomolecules. The optimisation of the used method is very important to maximise the process productivity. BioPro IEX SmartSep resins can be used up to 60°C - offering great flexibility in method development. This is particularly advantageous for the purification of oligonucleotides. 

Investigation of High Temperature Stability with Protein Samples

The examples shown in the Technical Note point demonstrate the extended temperature stability of the BioPro IEX SmartSep resins. In a first example, the BioPro IEX SmartSep S20 and Q20 resins were flushed with a buffer at 60 °C for 20 hours. The column performance test showed no retention time shift for the proteins investigated – even after repeating the procedure for 5 times! Furthermore, no change in the separation or peak distortion could be observed for BioPro IEX SmartSep Q and S resins even after 100 hours of exposure to a 60 °C solution.

More Practical Example for the Purification of Oligonucleotides

Even under more practical conditions for the purification of oligonucleotides the BioPro IEX SmartSep Q resin maintained its performance. The elution profile of an antisense oligonucleotide remained constant even after eluting the column at 60°C for 130 runs. There is no retention time shift, and the column efficiency remains the same.

Conclusion

These experiments demonstrate the excellent temperature stability of the BioPro IEX SmartSep resins. The resins maintain their performance when used with higher eluent temperatures. This can be useful for many applications such as oligonucleotide purifications and offer a greater flexibility in method development.
Find all these useful information about high temperature stability of BioPro IEX SmartSep resin in this Technical Note.
 


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