Outer membrane protein pattern examined in Salmonella enterica Hadar

Two-dimensional electrophoresis (2-DE) and LC-MS/MS spectrometry were used to explore the adaptation of salmonella enterica Hadar under static magnetic field to discover the effects on the outer membrane protein pattern.

By comparing the proteome of enriched-outer membrane fraction both before and after exposure to a magnetic field, researchers were able to identify protein patterns through a process of two-dimensional electrophoresis (2-DE) and LC-MS/MS spectrometry.

They found a total of 11 proteins (that displayed more than a two-fold change) were expressed differently in exposed cells. Of which, seven were up-regulated and four down-regulated.

Researchers then used the antibiotic disc diffusion method to confirm the decrease of gentamicin susceptibility in exposed cells. This may be partly associated with the up-regulation of TolC, outer membrane component of an efflux pump.

This showed that SMF (200 mT) maintains cell envelope integrity and exposes cells to oxidative stress. Some of the alterations show an ability of exposed cells to form biofilms.

Posted by Fiona Griffiths