• MALDI-TOF-MS a fast, reliable identification method

Electrophoretic Separations

MALDI-TOF-MS a fast, reliable identification method

Dec 23 2011

A recent study has concluded that Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) can be developed into a fast and reliable identification method for genetically highly related species when potential taxonomic and genetic inconsistencies are taken into account when generating a reference library.

The results showed that 99.3 per cent of the 152 tested were identified at the species level, and suis biovar 1 and 2 were identified at the level of their biovar. This demonstrates that for Brucella, even minimal genomic differences between these serovars translate to specific proteomic differences.

By constructing a Brucella reference library based on multilocus variable-number tandem repeat analysis (MLVA) data, an accurate identification of Brucella species using MALDI-TOF-MS was achievable. By comparing MS-spectra from Brucella species against a custom-made MALDI-TOF-MS reference library, MALDI-TOF-MS could be used as a rapid identification method for Brucella species.

The study cited MALDI-TOF-MS as a rapid, cost-effective, accurate and sustainable for the high-throughput identification of bacteria.

Digital Edition

Chromatography Today - Buyers' Guide 2022

October 2023

In This Edition Modern & Practical Applications - Accelerating ADC Development with Mass Spectrometry - Implementing High-Resolution Ion Mobility into Peptide Mapping Workflows Chromatogr...

View all digital editions


NGVS 2024

May 23 2024 Beijing, China


May 28 2024 Tel Aviv, Israel


May 28 2024 Leuven, Belgium

PREP 2024

May 28 2024 Philadelphia, PA, USA


May 29 2024 Beijing, China

View all events