• MALDI-TOF-MS a fast, reliable identification method

Electrophoretic separations

MALDI-TOF-MS a fast, reliable identification method

A recent study has concluded that Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) can be developed into a fast and reliable identification method for genetically highly related species when potential taxonomic and genetic inconsistencies are taken into account when generating a reference library.

The results showed that 99.3 per cent of the 152 tested were identified at the species level, and suis biovar 1 and 2 were identified at the level of their biovar. This demonstrates that for Brucella, even minimal genomic differences between these serovars translate to specific proteomic differences.

By constructing a Brucella reference library based on multilocus variable-number tandem repeat analysis (MLVA) data, an accurate identification of Brucella species using MALDI-TOF-MS was achievable. By comparing MS-spectra from Brucella species against a custom-made MALDI-TOF-MS reference library, MALDI-TOF-MS could be used as a rapid identification method for Brucella species.

The study cited MALDI-TOF-MS as a rapid, cost-effective, accurate and sustainable for the high-throughput identification of bacteria.


Events

ASMS Conference

Jun 01 2025 Baltimore, MD, USA

HPLC 2025

Jun 15 2025 Bruges, Belgium

LabAsia 2025

Jul 14 2025 Kuala Lumpur, Malaylsia

SinS Solutions in Science

Jul 15 2025 Brighton, UK

ACS National Meeting - Fall 2025

Aug 17 2025 Washington DC, USA & Virtual

View all events