Electrophoretic Separations
MALDI-TOF-MS a fast, reliable identification method
Dec 23 2011
A recent study has concluded that Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) can be developed into a fast and reliable identification method for genetically highly related species when potential taxonomic and genetic inconsistencies are taken into account when generating a reference library.
The results showed that 99.3 per cent of the 152 tested were identified at the species level, and suis biovar 1 and 2 were identified at the level of their biovar. This demonstrates that for Brucella, even minimal genomic differences between these serovars translate to specific proteomic differences.
By constructing a Brucella reference library based on multilocus variable-number tandem repeat analysis (MLVA) data, an accurate identification of Brucella species using MALDI-TOF-MS was achievable. By comparing MS-spectra from Brucella species against a custom-made MALDI-TOF-MS reference library, MALDI-TOF-MS could be used as a rapid identification method for Brucella species.
The study cited MALDI-TOF-MS as a rapid, cost-effective, accurate and sustainable for the high-throughput identification of bacteria.
Digital Edition
Chromatography Today - Buyers' Guide 2022
December 2021
In This Edition Modern & Practocal Applications - Accelerating ADC Development with Mass Spectrometry - Implementing High-Resolution Ion Mobility into Peptide Mapping Workflows Chromatogr...
View all digital editions
Events
Plastics Recycling World Expo Europe
Jun 14 2023 Essen, Germany
Jun 18 2023 Dusseldorf, Germany
Jul 04 2023 Cardiff, UK
Jul 11 2023 Lagos, Nigeria
ACS National Meeting & Expo, Fall 2023
Aug 13 2023 San Francisco, CA, USA