Electrophoretic Separations
Capto Blue affinity chromatography medium released
Dec 28 2011
GE Healthcare has released Capto Blue affinity chromatography medium, promoting the new product as being more chemically stable with a more rigid agarose base matrix than its predecessor, allowing for faster flow rates and larger sample volumes, which leads to higher throughput and improved process economy.
The chromatography medium has multiple purposes that include the capture of human serum albumin (HSA), as well as purification of HSA fusion proteins, blood coagulation factors, enzymes, and recombinant proteins at laboratory and process scales. It is suitable for the removal or purification of proteins at both laboratory and process scales.
Depending on the target molecule, the ligand functionality of Capto Blue may be modified through the use of appropriate buffer salts and buffer conductivity to increase selectivity for desired targets.
Capto Blue medium’s chemical stability also ensures tolerance for sodium hydroxide, enabling repeated cleaning-in-place (CIP) procedures to remove tightly bound impurities and maintain capacity.
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