The regulation of the expression of the enzyme class xylanase in Hypocrea jecorina has been studied by an international team using
analytical chemistry processes including electrophoresis.
A team at TU Wien and the Vienna Biocenter in Austria and at the University of Brasilia collaborated on the project, which is published in BMC Genomics.
H jecorina is important in hydrolase secretion, producing materials with applications in biofuels, paper,
food and textiles.
With high secretory capacity, it is used in industrial processes for protein production and fermentative generation of native extracellular enzymes.
The
analytical chemistry process combined electrophoresis with
high performance liquid chromatography and tandem mass spectrometry to identify transcription factors for the regulation of xylanase in H jecorina.
According to the researchers, a similar process could be applied to other experiments with high levels of throughput, providing relevant genome sequence databases are available.
BMC Genomics disseminates information on an open-access basis in all areas of functional genomics and proteomics.
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