Clean Columns. Clear Results. Discover the Stability of MacroSep IEX Q

Read YMC´s latest Technical Note about Cleaning-in-Place (CIP) stability of MacroSep IEX Q. The high-performance anion exchange resin designed for demanding tasks such as purification of plasmid DNA and viral vectors. In such applications, nonspecific adsorption and microbial contamination can quickly degrade chromatographic performance. That’s why CIP isn’t just a maintenance step – it’s essential for ensuring process reliability, reducing downtime, and maintaining product quality.

Why is CIP Important?

CIP is essential for the economic and efficient operation of packed chromatography columns. Effective cleaning procedures extend column lifetime, reduce overall process costs, and enhance downstream process safety and productivity. During the purification of viral vectors and large nucleic acids such as pDNA, nonspecific adsorption can lead to a loss in chromatographic performance. At the same time, microbial growth poses an additional risk, making regular CIP procedures essential to restore column performance and ensure sterility.

Routine CIP is essential to maintaining efficiency, sterility, and cost-effectiveness in packed column operations. It prevents:

• Loss of chromatographic performance due to contaminants
• Frit clogging and system pressure increases
• Microbial growth that can compromise product purity and safety

Sodium Hydroxide (NaOH) – The Industry Standard for CIP

The industry’s gold standard for CIP is 1 M Sodium Hydroxide (NaOH), known for its powerful cleaning and sanitising properties. It effectively removes contaminants, prevents frit clogging, and eliminates microbial contamination – all without the need to unpack the column.

To optimise process efficiency and reduce operational costs, chromatography resins must demonstrate high chemical stability under alkaline CIP conditions. MacroSep IEX Q from YMC meets these requirements and has been extensively tested for CIP stability.

Proven CIP Stability – Even After 200 Cycles

The CIP stability of MacroSep IEX Q was evaluated using 1 M NaOH in repeated cleaning cycles. The results clearly demonstrate that the resin is completely compatible with 1 M NaOH CIP protocols. Its high dynamic binding capacity is retained– even after 200 CIP cycles. This results in an extended column lifetime for cost-efficient purification. Whether you're optimising a new purification workflow or upgrading an existing one, MacroSep IEX Q ensures performance you can rely on.

Increased Column Lifetime & Process Efficiency

For efficient and economical IEX purification, effective CIP is essential. 1 M NaOH has been established as the industry standard, offering both impurity removal and column sanitisation. The chemical stability of the chromatography resin is a key factor in process efficiency and cost reduction. MacroSep IEX Q excels in alkaline CIP stability, ensuring long-term high performance of the column with reduced downtime due to consistent cleaning efficiency. This results in increased lifetime, even for challenging feed solutions.

Click here to download the full Technical Note to explore the study and results in detail. Want to see MacroSep IEX Q in action for yourself? Contact us today to request your sample and experience the benefits of robust alkaline CIP stability in your own lab.