High performance liquid chromatography has been used in the screening of soy protein-derived hypotriglyceridemic di-peptides.
In a study published by Lipids in Health and Disease, a team of scientists investigated the effects of soy protein-derived peptides on lipid metabolism - paying particular attention to triglyceride (TG) metabolism - in HepG2 cells and obese Otsuka Long-Evans Tokushima fatty (OLETF) rats.
The team undertook five separate experiments, the first two of which revealed that soy crude peptide (SCP)-LD3 showed hypolipidemic effects in HepG2 cells and OLETF rats and hydrophilic fraction, separated from SCP-LD3 with hydrophobic synthetic absorbent, revealed lipid-lowering effects.
Then, through a process involving gel permeation chromatography-high performance liquid chromatography, the team revealed that Fraction-C (Frc-C) peptides significantly reduced TG synthesis and apolipoprotein B (apoB) secretionin HepG2 cells.
As a result of all of the experiments, the scientists managed to isolate the novel active peptides with TG-lowering effects from soy protein.
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