HPLC, UHPLC
Liquid chromatography provides accurate Vitamin D measures
May 16 2011
Published by the Nutrition Journal, the study aimed to find a new way to detect Vitamin D as many commercially available immunoassays for the nutrient have been questioned with regard to accuracy.
Current methods for the detection of Vitamin D can be compromised by overlapping peaks and identical masses of epimers and isobars, resulting in inaccurate measurements.
A team of scientists comprising of Iltaf Shah, Ricky James, James Barker, Andrea Petroczi and Declan Naughton found that the new liquid chromatography-mass spectrometry method was capable of detecting 0.25 ng/mL of all analytes in serum.
The authors of the study told the source that this method is, to the best of their knowledge, the first reliable, reproducible and robust LC-MS/MS method for detecting low levels of Vitamin D.
They suggested that the analytical process will prove very useful to clinics as it does away with time consuming derivatisation and extraction techniques.
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