Scientists have used
liquid chromatography-mass spectrometry (LC-MS) methods to investigate sustained productivity in recombinant Chinese Hamster Ovary (CHO) cell lines.
In a study published by BMC Biotechnology, the team from the National Institute for Cellular Biotechnology at Dublin City University aimed to discover a molecular basis for sustainable productivity.
The scientists claimed that the ability of mammalian cell lines to sustain cell specific productivity over the full duration of bioprocess culture is a highly desirable phenotype.
Using LC-MS, the team highlighted 89 differently expressed proteins from CHO cell lines, with overlap comparisons between the two sets of comparative cell lines narrowing it down to just 12 proteins.
The study revealed that these proteins may have an important role to play in sustaining high productivity of recombinant protein over the duration of a fed-batch bioprocess culture.
"Moreover, it is possible that many of these proteins could be successfully manipulated or engineered so as to elicit the very phenotype we have characterised," the scientists claimed.
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