Solid Phase Extraction (SPE)
DNAzyme analysis indicates best quantities of enzyme and substrate
Dec 08 2010
A team from the University of Houston collaborated with local biotech firm BioTex to determine how DNAzymes - sequences of genetic code with enzymatic features - can be used with RNA substrates to manufacture specific sections of RNA.
They used a pair of biotinylated 8-17 DNAzymes to achieve this, excising 71-nucleotide recombinant RNA from a chimerical Vibrio proteolyticus gene transferred into E coli cells.
Ideal enzyme and substrate proportions were found to occur at a ratio of RNA substrate to enzymes of 1:10:10, with the latter figures relating to Pen17zyme1B and Pen17zyme2.
BMC Biotechnology specialises in studies of how experimental procedures may make use of organisms or biological macromolecules for pharmaceutical and agricultural biotechnology applications, as well as in allied industries.
Digital Edition
Chromatography Today - Buyers' Guide 2022
December 2021
In This Edition Modern & Practocal Applications - Accelerating ADC Development with Mass Spectrometry - Implementing High-Resolution Ion Mobility into Peptide Mapping Workflows Chromatogr...
View all digital editions
Events
Plastics Recycling World Expo Europe
Jun 14 2023 Essen, Germany
Jun 18 2023 Dusseldorf, Germany
Jul 04 2023 Cardiff, UK
Jul 11 2023 Lagos, Nigeria
ACS National Meeting & Expo, Fall 2023
Aug 13 2023 San Francisco, CA, USA