• NEW ACE® C18-PFP - a unique C18 bonded HPLC column with the extra selectivity of a pentafluorophenyl (PFP) phase
  • Separation Mechanism Table

Columns (LC)

NEW ACE® C18-PFP - a unique C18 bonded HPLC column with the extra selectivity of a pentafluorophenyl (PFP) phase

Feb 24 2011

ACE® C18-PFP is a unique C18 bonded HPLC column from Advanced Chromatography Technologies, which has been developed to provide alternate selectivity to all existing C18 bonded phases. The phase combines the hydrophobic characteristics of a C18 phase with the alternate selectivity offered by a pentafluorophenyl (PFP) phase – enabling the benefits of multiple interactions to be fully exploited.

ACE® C18-PFP also exhibits ultra low bleed to ensure LC/MS compatibility and demonstrates excellent temperature and pH stability. Compared to C18 phases, PFP phases have traditionally been compromised by reduced hydrophobicity, reduced stability and significant column bleed. However, ACE C18-PFP utilises a specially developed ligand combining a C18 chain with integral PFP functionality, resulting in a phase that maintains the hydrophobic, stability and low bleed characteristics of a leading C18 phase, whilst providing the multiple retention mechanisms of a PFP phase.

Due to their similar hydrophobic characteristics, ACE C18-PFP columns may be used for applications in which “standard” C18 columns would normally be considered. However, due to its integral pentafluorophenyl functionality, ACE C18-PFP is additionally recommended for separations that involve halogenated aromatic compounds, regioisomers and those analytes with differing shape constraints.

ACE® C18-PFP phase exhibits multiple retention mechanisms including hydrophobic,  interaction, dipole-dipole, hydrogen bonding and shape selectivity. Approximations of relative strength are shown in the accompanying table.

At launch, columns are available in 3µm, 5µm and 10µm particle sizes and a full range of dimensions from high throughput LC-MS through to preparative scale.
For further information and a Technical Brochure on the ACE® C18-PFP www.ace-hplc.com or email: info@ace-hplc.com

Digital Edition

Chromatography Today - Buyers' Guide 2022

December 2021

In This Edition Modern & Practocal Applications - Accelerating ADC Development with Mass Spectrometry - Implementing High-Resolution Ion Mobility into Peptide Mapping Workflows Chromatogr...

View all digital editions


Lab Asia 2023

Oct 10 2023 Hybrid event

MicroTAS 2023

Oct 15 2023 Katowice, Poland

ISPPP 2023

Nov 05 2023 Vienna, Austria

SETAC North America

Nov 12 2023 Louisville, KY, USA

Eastern Analytical Symposium

Nov 13 2023 Plainsboro, NJ, USA

View all events