Chromatography
Published over 14 years ago. See the latest and most current information on Chromatography.
Agilent Intelligent System Emulation Technology (ISET) is a function within the Agilent 1290 Infinity LC System that offers seamless transfer of methods from conventional LC systems such as the Agilent 1100 Series Quaternary LC System, which has a higher delay volume and different mixing behaviour, to the 1290 Infinity LC System. When the ISET function is enabled on the 1290 Infinity LC System, almost the same retention times and resolution can be obtained. This Technical Overview describes the transfer of a conventional HPLC method from a 1100 Series Quaternary LC System to the 1290 Infinity LC System. Metoclopramide and its impurities were analysed on both systems. Measurements were done using the 1290 Infinity LC System both with and without ISET enabled. To further improve agreement of retention times and resolution, the fine-tuning option of ISET was deployed. Retention times and resolution of the different experiments were evaluated and compared.
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