• Challenges during mAb purification

Preparative

Challenges during mAb purification

Nov 11 2016

For purification of monoclonal antibodies, high demands are required from the separating material. Factors influencing the binding characteristics of IgG are pH, linear velocity and/or salt concentration (conductivity) at the time the sample is loaded onto the column.

Therefore, a material with highly stable performance is required with regard to all those factors. In order to demonstrate the performance of YMC-BioPro materials, several studies have been performed.

High binding capacities are achieved regardless of elution of pH. Therefore, milder eluting conditions for Adalimumab can be selected.

BioPro SmartSep maintains higher binding capacity values over a wider range of linear velocity. This will increase product throughput for the purification work without loss of ef­ficiency.

BioPro SmartSep has higher salt concentration tolerance. This simplifies the desalting pro­cess after Protein A chromatography and will help to shorten the production process.

BioPro SmartSep materials meet the highest demands for the purification of monoclonal antibodies. High binding capacity is achieved regardless of elution of pH, linear velocity or salt concentration. This allows purification processes to be carried out more efficiently.

 www.ymc.de


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