High-Performance Large Molecule Separations with HALO® 1,000 Å Fused-Core® Columns

As the first manufacturer of the 1000 Å Fused-Core® particle, Advanced Materials Technology recognises the benefits of unrestricted pore access when analysing large biomolecules. HALO® BioClass 1000 Å phases have been developed to provide you with unique selectivities that can help you achieve your challenging separations easily.

HALO® BioClass 1000 Å, 2.7 µm phases are available in three chemistries: C4, ES-C18, and Diphenyl. Perfect for the separation of large biomolecules ≤ 500 kDa including monoclonal antibodies (mAbs), antibody-drug conjugates (ADCs), and biosimilars, HALO® Fused-Core® columns produce narrow peaks and the highest resolution thanks to their innovative phase access and leading pore size technology. Furthermore, HALO® 1000 phase yield higher recoveries versus smaller-pore phases and fully-porous molecules.

HALO® proven high efficiency Fused-Core® 2.7 µm columns offer comparable peak capacities to sub-2 µm fully-porous columns at 40–50% of the backpressure. These phases can offer UHPLC-like performance using conventional HPLC instrumentation. HALO® 1000 Å columns are manufactured to the highest quality standards, ensuring rugged column performance at low pH and high temperatures. HALO® columns are compatible with both HPLC and UHPLC instrumentation for rapid, high efficiency bioseparations.

Additionally, HALO® BioClass C4 and ES-C18 chemistries are also available with a pore size of 400 Å - perfect for the high efficiency analysis of your smaller proteins and biomolecules.

For further details on HALO® 1000 Å columns, information on any other HALO® product, or to discuss how to convert your HPLC method to an ultra-fast method using superficially-porous phases, contact the friendly and knowledgeable technical team at hichrom who will be happy to help.