Electrophoretic Separations
DNA secondary structure is influenced by genetic variation
Sep 13 2011
In a study published by Molecular Cytogenetics and undertaken by teams in Japan and the US, scientists sought to identify the way that DNA secondary structures impact DNA replication, transcription and genomic rearrangements.
One of the clearest and most known examples is the recurrent constitutional t(11;22) in humans that is mediated by potentially cruciform-forming sequences at the breakpoints, palindromic AT-rich repeats (PATRRs).
In a previous study, the team illustrated that polymorphisms of PATRR sequences affect the frequency of de novo t(11;22)s in sperm samples from healthy males, but this investigation aimed to determine if PATRR polymorphisms affect DNA secondary structure and therefore lead to variation in translocation frequency.
The scientists found that the propensity for DNA cruciform structure of each polymorphic allele correlates with the frequency of de novo t(11;22)s produced.
This strongly suggests that PATRR adopts unstable cruciform structures during spermatogenesis.
Digital Edition
Chromatography Today - Buyers' Guide 2022
October 2023
In This Edition Modern & Practical Applications - Accelerating ADC Development with Mass Spectrometry - Implementing High-Resolution Ion Mobility into Peptide Mapping Workflows Chromatogr...
View all digital editions
Events
May 05 2024 Seville, Spain
May 15 2024 Birmingham, UK
May 19 2024 Brno, Czech Republic
May 21 2024 Lagos, Nigeria
May 23 2024 Beijing, China