• Post-transitional modification found in muscle larvae


Post-transitional modification found in muscle larvae

Feb 13 2012

Two-dimensional difference gel electrophoresis and immunoblotting have been used to compare excretory-secretory antigens of Trichinella spiralis and Trichinella britovi muscle larvae, finding post-transitional modification to occur in some of the proteins.

The study was able to reveal positive results which highlighted the value of 2-D DIGE as a versatile tool to compare secretomes of different Trichinella species for pinpointing factors contributing to the interaction with the host. Both 2-D DIGE and 2-DE immunoblotting approaches indicated that T. spiralis and T.britovi produce somewhat distinctive antigen profiles, which contain E-S antigens with potential as species-specific diagnostic markers for Trichinella.

According to the analysis, 17 proteins could be identified from a total of 22 that had been subject to analysis, of many were identified in multiple spots, suggesting that they have undergone post-translational modification, possibly involving glycosylation or proteolysis.

The findings also suggest that some of the commonly identified proteins were post-translationally modified to different extents, which in certain cases seemed to result in species-specific modification.

Published by Ben Evans

Digital Edition

Chromatography Today - Buyers' Guide 2022

October 2023

In This Edition Modern & Practical Applications - Accelerating ADC Development with Mass Spectrometry - Implementing High-Resolution Ion Mobility into Peptide Mapping Workflows Chromatogr...

View all digital editions


MSB 2024

May 19 2024 Brno, Czech Republic

Water Expo Nigeria 2024

May 21 2024 Lagos, Nigeria

NGVS 2024

May 23 2024 Beijing, China


May 28 2024 Tel Aviv, Israel


May 28 2024 Leuven, Belgium

View all events