• Biologically active substances 'show greater variation' in human samples
    Human samples of biologically active substances offer greater variation, researchers note

Bioanalytical

Biologically active substances 'show greater variation' in human samples

Sep 06 2010

The analysis of biologically active substances in human test groups is more likely to yield a wide distribution with multiple differences and outlying results, research suggests.

Scientists at the University of Massachusetts Amherst, private health university UMIT and life sciences company BG Medicine examined the differences between human and animal results.

Their focus was on the assessment of biologically active substances using 'omics' - methods of analysis such as genomics and proteomics.

"Data measured in human studies were characterised by higher biological variation and the presence of outliers," they write.

Comparing different distributions with the classifiers commonly used in such research, they found balanced, Gaussian models most compatible with Prediction Analysis for Microarrays, or PAM.

Asymmetrical distributions and unbalanced class patterns were better assessed using Random Forests as the classifier, they add.

The -omics studies they considered had all made use of multiplexed immunoassay techniques and high-content mass spectrometry as part of highly dimensional data sets.

Digital Edition

Chromatography Today - Buyers' Guide 2022

December 2021

In This Edition Modern & Practocal Applications - Accelerating ADC Development with Mass Spectrometry - Implementing High-Resolution Ion Mobility into Peptide Mapping Workflows Chromatogr...

View all digital editions

Events

Lab Asia 2023

Oct 10 2023 Hybrid event

MicroTAS 2023

Oct 15 2023 Katowice, Poland

ISPPP 2023

Nov 05 2023 Vienna, Austria

SETAC North America

Nov 12 2023 Louisville, KY, USA

Eastern Analytical Symposium

Nov 13 2023 Plainsboro, NJ, USA

View all events