HPLC, UHPLC

Turning on the Heat in Liquid Chromatography - Tony Edge & Luisa Pereira

Sep 15 2010

Author: Tony Edge & Luisa Pereira on behalf of Thermo Fisher Scientific (UK) Ltd

Free to read

This article has been unlocked and is ready to read.

Download

The use of temperature within liquid chromatography (LC) has traditionally been limited to isothermal studies up to 50° or 60°C. However, this does not realise the full potential that temperature can have within a liquid chromatographic system. In particular the ability to run with thermal gradients [1,2] or to run green LC [3,4], where there is no organic solvent used, is something that is not considered as routine within the liquid chromatography community.

The advent of ultra-high pressure liquid chromatography (UHPLC) has meant that chromatographers are becoming more aware of the extremes of chromatography, and the benefits that this can have in terms of the separations either due to efficiency gains or reduction in sample analysis times. The advent of the new phases has also led coincidentally to robustness in the column performance at elevated temperature allowing for more extreme chromatography to be tried. The advantages of this form of extreme chromatography are many-fold e.g. a reduction in viscosity leads naturally to a reduction in the operating pressure, allowing for much higher flow rates and thus reducing analysis times. It also leads to substantial changes in selectivity which can benefit the chromatographer. Interestingly the use of HypercarbTM, which is an ideally suited stationary phase for these extreme conditions, is seeing an increase in the number of applications [5,6].

Free to read

This article has been unlocked and is ready to read.

Download


Digital Edition

Chromatography Today - Buyers' Guide 2022

December 2021

In This Edition Modern & Practocal Applications - Accelerating ADC Development with Mass Spectrometry - Implementing High-Resolution Ion Mobility into Peptide Mapping Workflows Chromatogr...

View all digital editions

Events

MC 2023

Feb 26 2023 Darmstadt, Germany

analytica China - NEW DATES

Mar 01 2023 Shanghai, China

China Lab 2023

Mar 09 2023 Guangzhou, China

PITTCON 2023

Mar 18 2023 Philadelphia, PA, USA

ACS National Meeting & Expo, Spring 2023

Mar 26 2023 Indianapolis, IN, USA

View all events