Replacing electrophoresis with pressure-driven flow
Jan 24 2012
Scientists at the French National Centre of Scientific Research's Laboratory for Analysis and Architecture of Systems (LAAS-CNRS) have postulated that replacing electrophoresis with pressure-driven flow could be more effective for separating DNA strands, according to Seperations Now.
According to the research, pumping the strands through nanopillar arrays by pressure allows for cleaner separations than those generated by electrophoresis. This overcomes electrophoresis limitations such as separating larger DNA strands and the separation of strands at small scales, such as those found on lab-on-a-chip devices.
The discovery showed that using single molecule fluorescence microscopy to monitor DNA migration through a nanopillar array made the DNA strands adopt unique configurations, judging by how they are propelled.
They also found that when propelled by electrophoresis, the strands were pulled off the nanopillars around four times faster than when propelled by pressure. The scientists separated two large DNA strands in only two minutes in testing, which compares to over an hour for conventional gel electrophoresis.
Posted by Fiona Griffiths
Chromatography Today - Buyers' Guide 2022
In This Edition Modern & Practocal Applications - Accelerating ADC Development with Mass Spectrometry - Implementing High-Resolution Ion Mobility into Peptide Mapping Workflows Chromatogr...
View all digital editions
Plastics Recycling World Expo Europe
Jun 14 2023 Essen, Germany
Jun 18 2023 Dusseldorf, Germany
Jul 04 2023 Cardiff, UK
Jul 11 2023 Lagos, Nigeria
ACS National Meeting & Expo, Fall 2023
Aug 13 2023 San Francisco, CA, USA