Electrophoretic Separations
Quantitative analysis with GE-MS deciphers wheat flour complexities
Feb 14 2011
Compiled by scientists at the USDA Agricultural Research Service's Western Regional Research Center, the quantitative analysis appear in Proteome Science, a periodical focusing on research into all aspects of both structural and functional proteomics.
They explain that a flour sample contains many proteins and, therefore, MS analysis alone has proved difficult in terms of relating each identified spot to a given gene sequence.
Using GE-MS to undertake quantitative analysis of wheat flour, however, the scientists were able to identify 233 protein spots, equivalent to 93.1 per cent of the normalised volume of their sample.
"The ability to measure expression levels for individual flour protein genes complements information gained from efforts to sequence the wheat genome and is essential for studies of effects of environment on gene expression," the researchers conclude in their report.
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