• MALDI-TOF instruments suitable alternatives to nano-LC-MS

Electrophoretic Separations

MALDI-TOF instruments suitable alternatives to nano-LC-MS

Jan 31 2012

The increased precision of MALDI-TOF instruments is making them into a suitable replacement for nano-LC-MS technology for detection in Stable Isotope Standards and Capture by Anti-Peptide Antibodies (SISCAPA) assays.

Bruker Corporation and SISCAPA Assay Technologies demonstrated that SISCAPA MALDI-TOF mass spectrometry can provide improved precision for the quantification of proteins such as high value protein biomarkers. The system was also found to be much easier and far more robust by researchers who will present their results in the Journal of Proteome Research.

MALDI-TOF are considered by many researchers to be lacking in precision, which makes it unsuitable for the quantitative analysis used in most clinical biomarker assays. However, MALDI-TOF technology has advanced in recent years and now includes many features that render this previously problematic state.

High repetition rate lasers (up to 1 kHz) and automated self cleaning ion sources are two such features that allow for faster results that can be maintained on a  24/7 basis. They have also been found to add much needed consistency.

Leigh Anderson, Ph.D, is the inventor of SISCAPA, and commented that: "Our paper demonstrates the very high precision of MALDI-TOF for peptide quantitation using internal standards, and establishes a solid basis for further exploring the utility of SISCAPA MALDI-TOF in biomarker verification and preclinical research."

Posted by Ben Evans


Digital Edition

Chromatography Today - Buyers' Guide 2022

October 2023

In This Edition Modern & Practical Applications - Accelerating ADC Development with Mass Spectrometry - Implementing High-Resolution Ion Mobility into Peptide Mapping Workflows Chromatogr...

View all digital editions

Events

analytica 2024

Apr 09 2024 Munich, Germany

Korea Lab 2024

Apr 23 2024 Kintex, South Korea

Korea Chem 2024

Apr 23 2024 Seoul, South Korea

AOCS Annual Meeting & Expo

Apr 28 2024 Montreal, Quebec, Canada

SETAC Europe

May 05 2024 Seville, Spain

View all events