• Low cost EV71 detection method developed
    Scientists have developed a new process to detect EV71 in clinical specimens.

Electrophoretic Separations

Low cost EV71 detection method developed

Jul 18 2011

Scientists have turned to quantitative analysis methods to develop a new technique for the detection of enterovirus 71 (EV71) in clinical specimens.

EV71 causes hand, foot, and mouth disease (HFMD) and is a common illness in China, with affected children and infants suffering from a fever, a blister-like reaction in the mouth and a skin rash.

The aim of the study was to develop a rapid, sensitive, specific and accurate method for the detection of EV71 in clinical specimens, as EV71 is often confused with coxsackievirus A16 (CVA16) infections and the present laboratory diagnosis of EV71 infection is time-consuming and costly.

Through the use of several quantitative analysis methods, the scientists identified and developed a one-step, single tube, reverse transcription-loop-mediated isothermal amplification (RT-LAMP) assay for rapid and sensitive detection of EV71.

In the report, the scientists claimed that the highly sensitive and rapid detection method has low costs, making it ideal for "primary health care institutions, field environments or developing countries".

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