• Electrophoresis used in quantitative analysis of IBD in chickens
    Gel electrophoresis has been applied to the quantitative analysis of the chicken virus IBD

Electrophoretic Separations

Electrophoresis used in quantitative analysis of IBD in chickens

Mar 09 2011

Gel electrophoresis is helping scientists in the quantitative analysis of infectious bursal disease (IBD), a virus which can be contracted by chickens.

The research is published in Virology Journal, a periodical dedicated to disseminating findings in all aspects of virus-borne disease.

In their paper, the scientists from the Chinese Academy of Agricultural Sciences explain how they used gel electrophoresis in the quantitative analysis of the detection limits involved in a one-step process for identifying the IBD virus.

The initial detection was carried out using reverse-transcription loop-mediated isothermal amplification (RT-LAMP), followed by a post-amplification restriction enzyme process to single out the most virulent strain.

"RT-LAMP is a rapid, simple and sensitive assay," the researchers write in their report.

"This method holds great promise, not only in laboratory detection and discrimination of the IBD virus, but also in large-scale field and clinical studies," they add.

According to their results, the RT-LAMP screening process is highly feasible for clinical applications, with 100 per cent correlation between its results and real-time polymerase chain reaction analysis.

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