Super heated water chromatography - a hot topic for LC and LC-MS

Oct 26 2009

Author: Tony Edge on behalf of Unassigned Independent Article

Free to read

This article has been unlocked and is ready to read.


This article discusses the use of high temperature water as a mobile phase and in particular to its application with sub 2 micron phases. A model is discussed which allows for the characterisation of the experimental conditions and is used later to determine the conditions required for an isobaric thermal gradient to be operated. The use of isothermal and thermal gradients are discussed and some example separations are given.

Water at room temperature is not commonly used as an elution solvent in reversed phase liquid chromatography, primarily because it does not elute most hydrophobic compounds. As a result organic solvents, such as methanol and acetonitrile, are routinely used to elute hydrophobic compounds from a hydrophobic stationary phase, either isocratically or by using a gradient. These solvents also have the advantage that they possess lower viscosity than water and as a result lower the operating pressure of the HPLC system. This has become of particular importance in recent years, with the advent of sub 2 micron particles.

Free to read

This article has been unlocked and is ready to read.


Digital Edition

Chromatography Today - Buyers' Guide 2022

October 2023

In This Edition Modern & Practical Applications - Accelerating ADC Development with Mass Spectrometry - Implementing High-Resolution Ion Mobility into Peptide Mapping Workflows Chromatogr...

View all digital editions


NGVS 2024

May 23 2024 Beijing, China


May 28 2024 Tel Aviv, Israel


May 28 2024 Leuven, Belgium

PREP 2024

May 28 2024 Philadelphia, PA, USA


May 29 2024 Beijing, China

View all events