The Application of Porous Graphitic Carbon to Proteomic Studies

Sep 18 2012

Author: by John R. Griffiths1, Valeria Barattini2, Joanna Freeke2, Duncan L. Smith1 on behalf of Thermo Fisher Scientific (UK) Ltd

Free to read

This article has been unlocked and is ready to read.


An investigation of Porous Graphitic Carbon (PGC) as a stationary phase for the analysis of proteomic samples in a two-dimensional liquid chromatography (2D-LC) workflow is presented. A selectivity study performed on a standard mixture containing 15 peptides shows that the elution order obtained using PGC deviates significantly from conventional reversed phase selectivity. Furthermore PGC has been shown to retain and resolve small polar peptides otherwise unretained on standard reversed phase C18 stationary phases. The separation mechanism orthogonality compared to C18 combined with greater retentivity for polar analytes makes the use of PGC in 2 dimensional separations ideal. An analysis of a whole cell lysate by PGC prefractionation has been shown to result in an increase of nearly 6000 unique peptides compared with SCX as the first dimension separation of a 2D-LC-MS/MS experiment.

Free to read

This article has been unlocked and is ready to read.


Digital Edition

Chromatography Today - Buyers' Guide 2022

October 2023

In This Edition Modern & Practical Applications - Accelerating ADC Development with Mass Spectrometry - Implementing High-Resolution Ion Mobility into Peptide Mapping Workflows Chromatogr...

View all digital editions


Water Expo Nigeria 2024

May 21 2024 Lagos, Nigeria

NGVS 2024

May 23 2024 Beijing, China


May 28 2024 Tel Aviv, Israel


May 28 2024 Leuven, Belgium

PREP 2024

May 28 2024 Philadelphia, PA, USA

View all events