HPLC, UHPLC
Protein Analysis using Proteotypic Peptides and LC-MS: Choosing the Chromatographic Separation for Optimal Coverage or Throughput
Oct 21 2009
Author: Chris Barton and Richard Kay on behalf of Quotient BioResearch Ltd
The science of protein analysis encompasses a range of analytical technologies including long established approaches such as gel electrophoresis and enzyme-linked immunosorbent assays (1, 2) Over the past ten years, mass spectrometry, linked with soft ionisation techniques such as electrospray ionisation (ESI), nanospray and matrix assisted laser desorption/ionisation (MALDI), has developed into an excellent tool for studying proteins in complex matrices. The combination of nanospray and liquid chromatography has since become the technique of choice for the majority of protein mass spectrometrists.
Digital Edition
Chromatography Today - Buyers' Guide 2022
October 2023
In This Edition Modern & Practical Applications - Accelerating ADC Development with Mass Spectrometry - Implementing High-Resolution Ion Mobility into Peptide Mapping Workflows Chromatogr...
View all digital editions
Events
Apr 09 2024 Munich, Germany
Apr 23 2024 Kintex, South Korea
Apr 23 2024 Seoul, South Korea
Apr 28 2024 Montreal, Quebec, Canada
May 05 2024 Seville, Spain