Protein Analysis using Proteotypic Peptides and LC-MS: Choosing the Chromatographic Separation for Optimal Coverage or Throughput

HPLC, UHPLC

Protein Analysis using Proteotypic Peptides and LC-MS: Choosing the Chromatographic Separation for Optimal Coverage or Throughput

21 Oct, 2009

Published over 16 years ago. See the latest and most current information on HPLC, UHPLC.

Chris Barton and Richard Kay
1 min read
Download

The science of protein analysis encompasses a range of analytical technologies including long established approaches such as gel electrophoresis and enzyme-linked immunosorbent assays (1, 2) Over the past ten years, mass spectrometry, linked with soft ionisation techniques such as electrospray ionisation (ESI), nanospray and matrix assisted laser desorption/ionisation (MALDI), has developed into an excellent tool for studying proteins in complex matrices. The combination of nanospray and liquid chromatography has since become the technique of choice for the majority of protein mass spectrometrists.

Latest Articles

Explore Our Other Sites

Labmate Online
analytica Vietnam 2027 returns to Ho Chi Minh City
Explore more Arrow
Envirotech Online
Mercury emissions monitoring for environmental compliance
Explore more Arrow
Pollution Solutions Online
Energy efficiency first: Why shipping must act now while low-GHG fuels scale
Explore more Arrow
Petro Online
Dr. Raj Shah receives NLGI Achievement Award, adding to a distinguished career in fuels, lubricants and petroleum testing
Explore more Arrow