Protein Analysis using Proteotypic Peptides and LC-MS: Choosing the Chromatographic Separation for Optimal Coverage or Throughput

HPLC, UHPLC

Protein Analysis using Proteotypic Peptides and LC-MS: Choosing the Chromatographic Separation for Optimal Coverage or Throughput

21 Oct, 2009

Published over 16 years ago. See the latest and most current information on HPLC, UHPLC.

Chris Barton and Richard Kay
1 min read
Download

The science of protein analysis encompasses a range of analytical technologies including long established approaches such as gel electrophoresis and enzyme-linked immunosorbent assays (1, 2) Over the past ten years, mass spectrometry, linked with soft ionisation techniques such as electrospray ionisation (ESI), nanospray and matrix assisted laser desorption/ionisation (MALDI), has developed into an excellent tool for studying proteins in complex matrices. The combination of nanospray and liquid chromatography has since become the technique of choice for the majority of protein mass spectrometrists.

Latest Articles

Explore Our Other Sites

Labmate Online
Portable fluorescence reader could bring forward molecular tests for use at home
Explore more Arrow
Envirotech Online
Advanced heated sampling probe enhances stack emissions monitoring
Explore more Arrow
Pollution Solutions Online
Leading UK biogas operator places first orders for new FlowSep technology
Explore more Arrow
Petro Online
Vietnam’s E10 rollout puts fuel testing capacity under closer scrutiny
Explore more Arrow