Advantages and Disadvantages of HILIC; a Brief Overview
May 31 2012 Read 8331 Times
Free to read
This article has been unlocked and is ready to read.
Hydrophilic interaction chromatography (HILIC) is fast becoming the preferred technique when encountered with polar and/or basic solutes. In comparison to reversed-phase, HILIC affords several advantages making the technique in such cases. In particular, the kinetic advantages yield much higher solute diffusivity, increased sensitivity with ESI-MS and highly symmetrical peak shapes.
Heavy reliance on the aprotic solvent acetonitrile can be viewed as the major disadvantage of HILIC predominantly from a sourcing perspective. Furthermore, in light of the highly valued and well understood method development associated with reversed-phase liquid chromatography it could be perceived that HILIC is a less flexible technique. Therefore while the HILIC mode of LC is being taken up on a more widespread basis, especially in the arena of drug and biological substance analysis, it is worthwhile to consider its basis, ad as well as some advantages and disadvantages of the technique, particularly in the arena of drug and biological substance analysis are presented.
In This Edition Articles Fundamental Aspects - Improved Biomacromolecule Separations Using Superficially Porous Particles with a 1000 Å Pore Diameter - A Deep Characterisation of Monoclo...
View all digital editions
Jan 30 2019 Amsterdam, Netherlands
Jan 31 2019 Paris, France
Feb 04 2019 Dubai, UAE
Feb 26 2019 Guangzhou, China
Mar 12 2019 Tel Aviv, Israel