Electrophoretic Separations

Analysis of Monoclonal Antibody Charge Variants by Capillary Zone Electrophoresis

Sep 18 2012

Author: by Marcia R Santos on behalf of Beckman Coulter Ltd

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Charge heterogeneity analysis is important in the characterisation of monoclonal antibodies because it provides important information about product quality and stability. Heterogeneity can be caused by such molecular adaptions as C-terminal lysine modification, deamidation, and post translational modification. One method for separating charge variants is capillary isoelectric focusing (cIEF) which provides information regarding isoelectric point variation for related molecular isoforms. cIEF requires electrophoretic separation in a coated capillary which helps suppress electro-osmotic flow (EOF) and prevents surface protein adsorption but can result in a fairly long cycle time and complex sample preparation. 

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