Electrophoretic Separations

Capillary Electrophoresis coupling to Mass Spectrometry (CE-MS), an advanced technique orthogonal to LC-MS for high resolution separation and accurate molecule identification - Martin Greiner

Dec 17 2010

Author: Martin Greiner on behalf of Agilent Technologies Europe

Free to read

This article has been unlocked and is ready to read.

Download

Capillary Electrophoresis (CE) coupled with Mass Spectrometry (MS) has been under investigation for the past couple of decades1. Combining the high separation power of CE with MS promises to yield more sensitive detection and higher information content on the analytes than current methods such as standard UV detection. Since those early days, there have been many improvements and technology advancements on CE and MS instruments, as well as the interfacing devices. Modern Time of Flight (TOF) MS instruments and robust commercial interfaces provide increasingly sensitive routine analysis with accurate mass data.

The basic approach to CE-MS is to use Electrospray Ionization (ESI) techniques. The advantage to this system is that typically ionized or polar compounds separated by the CE under low flow conditions (low nl/min range) can then be efficiently transferred from the liquid phase into gas phase ions required by MS instruments. In addition, ESI does not decompose fragile molecules, like proteins. Routine CE-MS requires the use of volatile
buffers or low concentration salts. This avoids contamination of MS instruments and the suppression of analyte ions by salts competing for entrance to the MS. Separation by CE and ionization of molecules in the ESI source are both based on analyte chemistry and require optimization for best results. It is critical to choose the correct solvents and pH ranges for solubility, to allow for optimal CE mobility and for high ionization efficiency during desolvation. This may call for different chemistries for the separation buffers and for a sheath liquid controlling the ESI processes.
 

Free to read

This article has been unlocked and is ready to read.

Download


Digital Edition

Chromatography Today - Buyers' Guide 2022

December 2021

In This Edition Modern & Practocal Applications - Accelerating ADC Development with Mass Spectrometry - Implementing High-Resolution Ion Mobility into Peptide Mapping Workflows Chromatogr...

View all digital editions

Events

ICMGP 2022

Jul 24 2022 Virtual event

ACS National Meeting & Expo, Fall 2022

Aug 21 2022 Chicago, IL, USA & Online

ACHEMA 2022 - NEW DATES

Aug 22 2022 Frankfurt, Germany

IMSC 2022

Aug 27 2022 Maastricht, Netherlands

EuCheMS Chemistry Congress

Aug 28 2022 Lisbon, Portugal

View all events