• Receptor-influenced protein studied in breast cancer using affinity chromatography
    The receptor-influenced protein GST can be examined using affinity chromatography, researchers confirm

Solid Phase Extraction (SPE)

Receptor-influenced protein studied in breast cancer using affinity chromatography

Aug 24 2010

Scientists at the Czech Institute of Organic Chemistry and Biochemistry have used affinity chromatography to assess levels of glutathione S-transferases (GSTs) in breast cancer patients, proteins known to inversely correlate with the oestrogen receptor.

Their studies focused on changes in expression of GSTs, which may be brought about by modified oestrogen receptor levels through the two substances' negative correlation with one another.

Affinity chromatography was used to separate glutathione-binding proteins from cell lines obtained in samples of breast tissue.

Meanwhile, two-dimensional electrophoresis was also used to compare the phenotypes of the cell lines.

"Our results confirmed the applicability of targeted affinity chromatography to proteome profiling and allowed us to characterise the phenotypes of four breast cancer cell lines," the researchers write in BMC Cancer 2010.

The electrophoresis revealed low correlation coefficients between cells of different types, indicating dissimilarity between the lines being examined, they add.

Digital Edition

Chromatography Today - Buyers' Guide 2022

October 2023

In This Edition Modern & Practical Applications - Accelerating ADC Development with Mass Spectrometry - Implementing High-Resolution Ion Mobility into Peptide Mapping Workflows Chromatogr...

View all digital editions

Events

China Lab 2024

Mar 05 2024 Guangzhou, China

analytica 2024

Apr 09 2024 Munich, Germany

Korea Lab 2024

Apr 23 2024 Kintex, South Korea

Korea Chem 2024

Apr 23 2024 Seoul, South Korea

AOCS Annual Meeting & Expo

Apr 28 2024 Montreal, Quebec, Canada

View all events