• Ion-exchange chromatography used to map arthritis epitopes
    Arthritis epitopes have been mapped using ion-exchange chromatography

Ion-exchange chromatography used to map arthritis epitopes

Dec 29 2010 Read 1342 Times

Citrullinated fibrinogen B-cell epitopes have been mapped in rheumatoid arthritis by scientists working at a number of Dutch institutions using ion-exchange chromatography.

A team from IBIS Technologies, Utrecht University, the University of Twente and Radboud University applied ion-exchange chromatography to fractionating peptides obtained from human fibrinogen citrullinated in vitro.

They suggest that patient sera offer a "versatile system for mapping modified amino acid-containing epitopes".

In all, 98 rheumatoid arthritis sera were analysed using the methodology devised by the scientists, which incorporates ion-exchange chromatography and high-resolution tandem mass spectrometry.

Their findings are published in the latest issue of Arthritis Research & Therapy, whose aim is to translate research into clinical practice.

According to the scientists, testing for the presence of autoantibodies offers a useful way to diagnose rheumatoid arthritis in human patients.

Citrullinated fibrinogen in particular is not a well understood issue, but has been linked with induced arthritis in mice, they add.ADNFCR-3194-ID-800314435-ADNFCR
Read comments0

Do you like or dislike what you have read? Why not post a comment to tell others / the manufacturer and our Editor what you think. To leave comments please complete the form below. Providing the content is approved, your comment will be on screen in less than 24 hours. Leaving comments on product information and articles can assist with future editorial and article content. Post questions, thoughts or simply whether you like the content.

Digital Edition

Chromatography Today - March 2019

March 2019

In This Edition Articles - Capillary Electrophoresis-Mass Spectrometry for Micro-Metabolomics - Imaged Capillary Iso-Electric Focussing: Background, Status, and Perspectives - High-Throughp...

View all digital editions


MSB 2019

Mar 25 2019 Corvallie, OR, USA

Forum Labo Paris

Mar 26 2019 Paris, France

ChemBio Finland

Mar 27 2019 Helsinki, Finland

ACS National Meeting & Expo

Mar 31 2019 Orlando, FL, USA

Food Science 2019

Apr 15 2019 Paris, France

View all events